Guide to Impedance Flow Cytometry: A Precise Method for Measuring Bacterial Numbers

Impedance flow cytometry is a newer, more precise method for measuring the number of bacteria in a sample. It works by passing an electrical current through the sample and measuring the resistance to flow (impedance). The higher the resistance, the greater the number of bacteria present.

The benefits over other methods:

This method has several advantages over traditional methods, such as culture-based methods.

  • First, it is much faster, giving results in minutes rather than days or weeks.
  • Second, it can be used to measure a wider range of bacterial numbers, from a few hundred to several million.
  • Finally, it is less likely to miss bacteria that are present at low levels.
  • As a result, impedance flow cytometry is becoming increasingly popular for applications such as monitoring water quality and detecting foodborne pathogens.

How to get started with impedance flow cytometry:

Cell-based assays are powerful tools for investigating the interactions between cells and their environment. Impedance flow cytometry is a type of cell-based assay that measures cell-substrate interactions in real-time.

By measuring the electrical impedance of cells as they Flow over a substrate, impedance flow cytometry can be used to quantify cell adhesion, cell spreading, and other cellular responses. Impedance flow cytometry can be used to monitor cells in 3D scaffolds and on complex substrates such as extracellular matrix proteins.

  • To get started with impedance flow cytometry, you will need a cell-based assay system that is equipped with an electrical impedance sensor.
  • Once you have your system set up, you will need to choose a substrate and prepare your cells for analysis.
  • Finally, you will need to run your assay and analyze your data.
  • With a little practice, you will be able to use impedance flow cytometry to study a variety of cell-substrate interactions.

Applications of impedance flow cytometry:

  • Impedance flow cytometry is a type of cytometry that uses an alternating current (AC) to measure cell size, granularity, and other physical properties.
  • Impedance flow cytometry is used in a variety of scientific fields, including medical diagnoses, cell physiology research, and cancer treatment.


It has many advantages over traditional methods of cell analysis, including its high throughput, low sample volume requirements, and its ability to measure multiple parameters simultaneously. In addition, impedance flow cytometry is non-destructive and can be used on live cells. As a result, it is an important tool for scientists who study the function of cells and the nature of diseases.